Journal: Frontiers in Chemistry

Article Title: Probing Multivalent Carbohydrate-Protein Interactions With On-Chip Synthesized Glycopeptides Using Different Functionalized Surfaces

doi: 10.3389/fchem.2021.766932

Figure Lengend Snippet: Fluorescence staining intensities of respective sugar azides 3 , 4 , 5 , 7 and 9 with their corresponding lectins: (A) α-Man azide 3 with ConA (CF ® 633 labeled, 100 μg/ml concentration), (B) β-GalNAc-PEG3 azide 7 with SBA (rhodamine labeled, 10 μg/ml concentration) (C) β-Gal azide 4 with RCA-I (rhodamine labeled, 10 μg/ml concentration), (D) β-Gal-PEG3 azide 5 with RCA-I (rhodamine labeled, 10 μg/ml concentration), (E) β-GlcNAc-PEG3azide 9 with WGA (rhodamine labeled) with 10 μg/ml concentration, and (F) 0.2 μg/ml concentration on PEPperPRINT slides with PEG-spacer (PPP-spacer; red), and on PolyAn functionalized slides with (dark blue) and without PEG-spacer (light blue).

Article Snippet: Amine functionalized glass slides from PEPperPRINT (PPP) were used with prior functionalization with a PEG-based spacer ( ).

Techniques: Fluorescence, Staining, Labeling, Concentration Assay

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Steps of chemical modification of an amine functionalized glass slide to produce an isocyanate functionalized slide for SMM fabrication.

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques: Modification

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: ( a ) After reaction with anti-biotin antibody, the change in OI-RD image (differential OI-RD image) of a biotinylated compound microarray on hexyl-isocyanate functionalized slide with (PEG) 6 and catalyzed in pyridine vapor at room temperature; ( b ) Extracted immobilization efficiencies of five biotinylated compounds with different nucleophilic residues available for surface anchoring.

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques: Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Immobilization efficiencies of biotinylated compounds on hexyl-isocyanate functionalized slide with spacers (PEG) n of varying lengths (n = 1, 2, 6, 12, 24, 36).

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Contact angles of DMSO on hexyl-isocyanate functionalized surface with spacers (PEG) n of varying lengths (n = 1, 2, 6, 12, 24, 36).

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Immobilization efficiencies of five biotinylated compounds with different nucleophilic residues printed on a phenyl-isocyanate functionalized surface as functions of post-printing treatment.

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Immobilization efficiencies of five biotinylated compounds with different nucleophilic residues printed on a hexyl-isocyanate functionalized slide followed by catalyzation in pyridine vapor at room temperature vs. those printed on phenyl-isocyanate functionalized slide followed by thermal annealing at 45 °C for 24 h.

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry

doi: 10.3390/s16030378

Figure Lengend Snippet: Combined auto-fluorescence/OI-RD image of a printed-and-washed compound microarray ( a ) on a hexyl-isocyanate functionalized slide and ( b ) on a phenyl-isocyanate functionalized slide.

Article Snippet: Amine functionalized glass slides were purchased from CapitalBio Corporation (Beijing, China).

Techniques: Fluorescence, Microarray